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TRXR E.coli Protein

CAT: 0247-OPPA02248-5UGSize: 5 µgDry Ice: NoHazardous: No
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CAT#:0247-OPPA02248-5UGSize:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Gene Aliases
TRXB, TRXR, Thioredoxin Reductase, TRXR E.Coli, Thioredoxin Reductase E.Coli Recombinant
Gene ID
12932721
Accession Number
NP_415408.1
Reactivity
Escherichia coli
Target
TRXR is a ubiquitous enzyme which participates in various cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase cleaves thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxidec
Type
Protein
Source
E. coli
Sequence
MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K.
Purification
TRXR protein is purified by standard chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Bioactivity
Specific activity is 4-5 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
Format
TRXR E.coli solution containing 20mM Tris HCl pH-8, 1mM DTT, and 10% glycerol. Physical appearance: Sterile filtered colorless solution.
Reconstitution
TRXR although stable at 4C for 1 week, should be stored desiccated below -18C. Please prevent freeze thaw cycles.
Notes
Formerly GWB-BSP324
Protein Length
Recombinant
NCBI Gene Symbol
TRXR
Protein Name
Thioredoxin reductase
Gene Name URL
TRXR