TEV Protein

• Gene Aliases:

  RTEV, TEV, P1 protease, TEV, Tobacco Etch Virus Protease Recombinant

• Reactivity:

  Tobacco Etch Virus

• Target:

  TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV) . Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q- (G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.

• Type:

  Protein

• Source:

  E. Coli

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Format:

  The rTEV contains 0.50 M Tris-HCl pH 8.0, 10mM DTT and 5 mM EDTA. Physical appearance: Sterile liquid formulation.

• Reconstitution:

  Store rTEV at -70C for long term or at -20C for< 6 months. Please prevent freeze-thaw cycles.

• Notes:

  Formerly GWB-92164F

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  TEV

• Protein Name:

  P1 protease EMBL ABI30960.1

• Gene Name URL:

  TEV