MAPK1 Protein

• Gene Aliases:

  ERK, p38, p40, p41, ERK2, ERT1, NS13, ERK-2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, p42-MAPK

• Gene ID:

  5594

• Accession Number:

  NP_002736.3

• Reactivity:

  Human

• Target:

  Mitogen-activated protein kinase 1 (MAPK1) is also known as "extracellular signal-regulated kinase 2" (ERK2) . Two similar (85% sequence identity) protein kinases were originally called ERK1 and ERK2. They were found during a search for protein kinases that are rapidly phosphorylated after activation of cell surface tyrosine kinasessuch as the epidermal growth factor receptor. Phosphorylation of ERKs leads to the activation of their kinase activity._x005F The molecular events linking cell surface receptors to activation of ERKs are complex. It was found that RasGTP-binding proteins are involved in the activation of ERKs. Another protein kinase, Raf-1, was shown to phosphorylate a "MAPK kinase", thus qualifying as a "MAPK kinase kinase". The MAPK kinase was named "MAPK/ERK kinase" (MEK) . Receptor-linked tyrosine kinases, Ras, Raf, MEK and MAPK could be fitted into a signaling cascade linking an extracellular signal to MAPK activation.Transgenic gene knockoutmice lacking MAPK1 have major defects in early development. MAPK1 Human Recombinant is a non-glycosylated full length chain containing amino acids Met1-Ser360.

• Type:

  Protein

• Sequence:

  Maaaaaagag pemvrgqvfd vgprytnlsy igegaygmvc saydnvnkvr vaikkispfe hqtycqrtlr eikillrfrh eniigindii raptieqmkd vyivqdlmet dlykllktqh lsndhicyfl yqilrglkyi hsanvlhrdl kpsnlllntt cdlkicdfgl arvadpdhdh tgflteyvat rwyrapeiml nskgytksid iwsvgcilae mlsnrpifpg khyldqlnhi lgilgspsqe dlnciinlka rnyllslphk nkvpwnrlfp nadskaldll dkmltfnphk rieveqalah pyleqyydps depiaeapfk fdmelddlpk eklkelifee tarfqpgyrs

• Purification:

  MAPK1 is purified by proprietary chromatographic techniques.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  326 ug/ml

• Bioactivity:

  M:

• Format:

  Liquid// 50mM HEPES (pH 7.5) with 100mM NaCl, 5mM DTT, and 20% glycerol Physical Appearance:// Sterile filtered clear solutionPhysical Appearance:// Sterile filtered clear solution

• Reconstitution:

  Store vial at -20C to -80C. Avoid multiple freeze-thaw cycles.

• Molecular Weight:

  42 kDa

• Notes:

  Formerly GWB-80DF6C

• Protein Length:

  Active|Recombinant

• NCBI Gene Symbol:

  MAPK1 ACTIVE

• Host or Source:

  E. Coli

• Protein Name:

  Mitogen-activated protein kinase 1

• Gene Name URL:

  MAPK1 Active