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RANBP2 Recombinant Protein (Human)

CAT: 0247-OPED00123-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OPED00123-100UGSize:100 µg
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CAS Number
9000-83-3
Gene Name
RAN binding protein 2
Gene Aliases
358 kDa nucleoporin; acute necrotizing encephalopathy 1 (autosomal dominant) ; ADANE; ANE1; E3 SUMO-protein ligase RanBP2; E3 SUMO-protein transferase RanBP2; IIAE3; nuclear pore complex protein Nup358; nucleoporin 358; nucleoporin Nup358; NUP358; P270; ran-binding protein 2; transformation-related protein 2; TRP1; TRP2.
Gene ID
5903
Accession Number
NP_060095.2
Reactivity
Homo sapiens|Human
Target
E3 SUMO-protein ligase which facilitates SUMO1 and SUMO2 conjugation by UBE2I (PubMed:11792325, PubMed:12032081, PubMed:15378033, PubMed:22194619, PubMed:15931224) . Involved in transport factor (Ran-GTP, karyopherin) -mediated protein import via the F-G repeat-containing domain which acts as a docking site for substrates (PubMed:7775481) . Binds single-stranded RNA (in vitro) (PubMed:7775481) . May bind DNA (PubMed:7775481) . Component of the nuclear export pathway (PubMed:10078529) . Specific docking site for the nuclear export factor exportin-1 (PubMed:10078529) . Sumoylates PML at 'Lys-490' which is essential for the proper assembly of PML-NB (PubMed:22155184) . Recruits BICD2 to the nuclear envelope and cytoplasmic stacks of nuclear pore complex known as annulate lamellae during G2 phase of cell cycle (PubMed:20386726) . Probable inactive PPIase with no peptidyl-prolyl cis-trans isomerase activity (PubMed:20676357, PubMed:23353830) .
Type
Protein
Purification
Purified by GST-based chromatography.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid. In 20mM HEPES, pH7.3 containing 110mM potassium acetate, 2mM magnesium acetate, 0.8mM EGTA, 1mM DTT.
Reconstitution
Store at -80C
Molecular Weight
59 kDa
Protein Length
Recombinant
NCBI Gene Symbol
RANBP2
Host or Source
E. Coli
Protein Name
E3 SUMO-protein ligase RanBP2
Gene Name URL
RANBP2
Nucleotide Accession Number
NM_017625.2