PARP1 Recombinant Protein

• Gene Aliases:

  ADP-ribosyltransferase diphtheria toxin-like 1; NAD (+) ADP-ribosyltransferase 1; Poly[ADP-ribose] synthase 1.

• Reactivity:

  Mouse|Mus musculus

• Target:

  Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP-ribosyl) ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity. Mediates the poly (ADP-ribosyl) ation of histones in a HPF1-dependent manner. Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming.

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  29kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  PARP1

• Host or Source:

  E.coli

• Protein Name:

  Poly [ADP-ribose] polymerase 1

• Gene Name URL:

  PARP1

• CAS Number:

  9000-83-3