FBXW7 Recombinant Protein

• Gene Name:

  F-box and WD-40 domain protein 7

• Gene Aliases:

  1110001A17Rik; A; AGO; Cdc4; F-box and WD-40 domain protein 7, archipelago homolog; F-box and WD-40 domain-containing protein 7; f-box only protein 30; F-box protein FBW7; F-box protein Fbxw6; F-box/WD repeat-containing protein 7; F-box-WD40 repeat protein 6; Fbw7; Fbwd6; Fbx30; Fbxo3; Fbxo30; Fbxw6; SEL-; SEL-10.

• Gene ID:

  50754

• Accession Number:

  NP_536353.2

• Reactivity:

  Mouse|Mus musculus

• Target:

  Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:21953459) . Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (By similarity) . Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1 (By similarity) . Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (By similarity) . SCF (FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1 (PubMed:21953459) . Involved in bone homeostasis and negative regulation of osteoclast differentiation (PubMed:29149593) .

• Conjugation:

  Unconjugated

• Type:

  Protein

• Source:

  E.coli

• Applications:

  Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  200 ug/mL (prior to lyoph)

• Format:

  Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.

• Reconstitution:

  2°C to 8°C|-80°C

• Molecular Weight:

  29.49kDa

• Protein Length:

  Recombinant

• NCBI Gene Symbol:

  Fbxw7

• Host or Source:

  E.coli

• Protein Name:

  F-box/WD repeat-containing protein 7

• Gene Name URL:

  Fbxw7

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_080428.3