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DLD Recombinant Protein

CAT: 0247-OPCD02757-200UGSize: 200 µgDry Ice: NoHazardous: No
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CAT#:0247-OPCD02757-200UGSize:200 µg
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CAS Number
9000-83-3
Gene Name
Dihydrolipoamide dehydrogenase
Gene Aliases
Diaphorase; Dihydrolipoamide dehydrogenase; dihydrolipoyl dehydrogenase, mitochondrial; DLDD; DLDH; E3; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; epididymis secretory sperm binding protein; GCSL; glycine cleavage system L protein; glycine cleavage system protein L; LAD; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; OGDC-E3; PHE3.
Gene ID
1738
Accession Number
NP_000099.2
Reactivity
Homo sapiens|Human
Target
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex) (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:17404228, PubMed:20160912, PubMed:20385101) . The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711) . A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711) . In monomeric form may have additional moonlighting function as serine protease (PubMed:17404228) . Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity) .
Conjugation
Unconjugated
Type
Protein
Source
E.coli
Applications
Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
200 ug/mL (prior to lyoph)
Format
Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Reconstitution
2°C to 8°C|-80°C
Molecular Weight
30kDa
Protein Length
Recombinant
NCBI Gene Symbol
DLD
Host or Source
E.coli
Protein Name
Dihydrolipoyl dehydrogenase, mitochondrial
Gene Name URL
DLD
Nucleotide Accession Number
NM_000108.4

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