Ripk3 Recombinant Protein

• Gene Name:

  Receptor-interacting serine-threonine kinase 3

• Gene Aliases:

  2610528K09Rik; AW107945; mRIP3; R; receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; Rip3; RIP-3; RIP-like protein kinase 3.

• Gene ID:

  56532

• Accession Number:

  NP_064339.2

• Reactivity:

  Mouse|Mus musculus

• Target:

  Essential for necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members. Upon induction of necrosis, RIPK3 interacts with, and phosphorylates RIPK1 and MLKL to form a necrosis-inducing complex. RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL. These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production.

• Type:

  Protein

• Source:

  Yeast

• Purification:

  Affinity purified using IMAC

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  Varies by lot. See vial for concentration.

• Format:

  Liquid// 20mM Tris-HCl, 0.5M NaCl (pH 8.0), 50% glycerol

• Reconstitution:

  Store at -20° C; for extended storage, conserve at -20° C or -80° C. Store at 4° C for up to one week. Repeated freeze/thaw cycles are not recommended.

• Molecular Weight:

  55 kDa

• Protein Length:

  1-486aa

• NCBI Gene Symbol:

  Ripk3

• Protein Name:

  Receptor-interacting serine/threonine-protein kinase 3

• Gene Name URL:

  Ripk3

• CAS Number:

  9000-83-3

• Nucleotide Accession Number:

  NM_019955.2