RIPK3 ELISA Kit (Human)

CAT: 0247-OKEH07133-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07133-96WSize:96 Tests
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Receptor interacting serine/threonine kinase 3
Gene Aliases
Receptor interacting protein 3; Receptor-interacting protein 3; receptor-interacting serine/threonine-protein kinase 3; RIP3; RIP-3; RIP-like protein kinase 3.
Gene ID
11035
Accession Number
NP_006862
Reactivity
Homo sapiens, Human
Target
Receptor-interacting serine/threonine-protein kinase 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology RIPK3 ELISA Kit (Human) (OKEH07133) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RIPK3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RIPK3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RIPK3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into matrix = 83%
Sensitivity
0.086 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Receptor-interacting serine/threonine-protein kinase 3
NCBI Gene Symbol
RIPK3
Protein Name
Q9Y572
Gene Name URL
RIPK3
Nucleotide Accession Number
NM_006871

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