BotA Recombinant Protein

CAT: 0247-OPCA02868-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0247-OPCA02868-20UGSize:20 µg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
CAS Number
9000-83-3
Gene Name
Botulinum neurotoxin type A
Gene Aliases
Bontoxilysin-A; botulinum neurotoxin type A; Botulinum neurotoxin type A1; CBO_RS04150; CBO0806.
Gene ID
5185061
Accession Number
WP_003356619.1
Reactivity
Clostridium botulinum
Target
Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure (PubMed:15394302, PubMed:7578132) . Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol (PubMed:17666397, PubMed:19096517) . Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release. Toxin activity requires polysialylated gangliosides; GT1b supports activity better than GD1a (PubMed:12089155) . Binds to host peripheral neuronal presynaptic membranes via the synaptic vesicle glycoproteins SV2A, SV2B and SV2C (PubMed:16543415) . It binds directly to the largest lumenal (intravesicular) loop of SV2A, SV2B and SV2C that is transiently exposed outside of cells during exocytosis; gangliosides enhance binding (PubMed:16543415, PubMed:16545378, PubMed:18815274) . Recognizes an N-linked glycan on SV2 proteins (PubMed:18815274, PubMed:27294781) . May also use FGFR3 as a receptor (PubMed:23696738) . Toxin uptake into neural cells requires stimulation (incubation with K (+) to stimulate receptor exposure) to be internalized by receptor-mediated endocytosis (PubMed:16543415, PubMed:19650874, PubMed:21632541, PubMed:21832053) . Subsequently the toxin colocalizes with its receptor in host cells (PubMed:16543415, PubMed:19650874) . Toxin uptake can be blocked by the appropriate SV2 protein fragments in cell culture (PubMed:16543415) .
Type
Protein
Source
Yeast
Sequence
MPFVNKQFNYKDPVNGVDIAYIKIPNVGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLLCVRGIIT
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
-20°C or -80°C
Molecular Weight
52 kDa
Protein Length
Recombinant
NCBI Gene Symbol
Bont
Host or Source
Clostridium botulinum
Protein Name
Botulinum neurotoxin type A
Gene Name URL
Bont
Nucleotide Accession Number
NZ_JFGN01000216.1

Related Products

CatalogName
OAMA02152-1MLBotA Antibody
OAMA02151-1MLOAMA02151-1ML - botA Antibody
OPCA02909-20UGBotF Recombinant Protein
OPCA02878-20UGBotD Recombinant Protein
OPCA02860-20UGBotB Recombinant Protein
OPCA02878-100UGBotD Recombinant Protein
OPCA02909-100UGBotF Recombinant Protein
OPCA02860-100UGBotB Recombinant Protein
OPCA02878-500UGOPCA02878-500UG - botD Recombinant Protein
OPCA02860-500UGOPCA02860-500UG - botB Recombinant Protein

Alternative Products

CatalogName
JN888012-01Clostridium botulinum BotA Recombinant Protein (N-His)
YXX00901Recombinant Clostridium botulinum botA/BOTOX Protein, C-His
MBS1568423-01Recombinant Anti-botA/BOTOX antibody
CSB-EP320799CLQ-01Recombinant Clostridium botulinum Botulinum neurotoxin type A (botA), partial
A134587Anti-botA/BoNT (Recombinant Human, Monoclonal, VHH-Fc)
AP72892Recombinant Clostridium botulinum Botulinum neurotoxin type A (botA) , partial
JN888263-01Anti-Clostridium botulinum botA/BoNT Recombinant Antibody (F12)
A133866Anti-botA/BOTOX (Recombinant Alpaca, Monoclonal, VHH-His)
JN888293-01Anti-Clostridium botulinum botA/BoNT Recombinant Nanobody (H7)
JN888283-01Anti-Clostridium botulinum botA/BoNT Recombinant Nanobody (F12)

Popular Products