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NfsA Recombinant Protein

CAT: 0247-OPCA02020-1MGSize: 1 mgDry Ice: NoHazardous: No
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CAT#:0247-OPCA02020-1MGSize:1 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E.coli.
CAS Number
9000-83-3
Gene Name
NADPH-dependent nitroreductase NfsA
Gene Aliases
B0851; ECK0842; mda18; mdaA; Modulator of drug activity A; NADPH-dependent nitroreductase NfsA; ybjB.
Gene ID
945483
Accession Number
NP_415372.1
Reactivity
Escherichia coli
Target
Catalyzes the reduction of nitroaromatic compounds using NADPH. Has a broad electron acceptor specificity. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major oxygen-insensitive nitroreductase in E.coli.
Type
Protein
Source
E.coli
Sequence
MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR
Purification
Affinity purified using IMAC
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Concentration
Varies by lot. See vial for concentration.
Format
Liquid or Lyophilized powder
Reconstitution
-20°C or -80°C
Molecular Weight
42.8 kDa
Protein Length
Recombinant
NCBI Gene Symbol
NfsA
Host or Source
Escherichia coli
Protein Name
Oxygen-insensitive NADPH nitroreductase
Gene Name URL
NfsA
Nucleotide Accession Number
NC_000913.3

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