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DegP Recombinant Protein

CAT: 0247-OPCA01983-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0247-OPCA01983-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
9000-83-3
Gene Name
Periplasmic serine endoprotease DegP
Gene Aliases
B0161; ECK0160; Heat shock protein DegP; htrA; periplasmic serine endoprotease DegP; Protease Do; ptd.
Gene ID
947139
Accession Number
NP_414703.1
Reactivity
Escherichia coli
Target
DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures (PubMed:10319814) . Degrades transiently denatured and unfolded or misfolded proteins which accumulate in the periplasm following heat shock or other stress conditions (PubMed:16303867) . DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids (PubMed:8830688) . Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins (PubMed:8830688) . Its proteolytic activity is essential for the survival of cells at elevated temperatures (PubMed:7557477) . It can degrade IciA, Ada, casein, globin and PapA. DegP shares specificity with DegQ (PubMed:8830688) . DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP) .
Type
Protein
Source
E.coli
Sequence
AETSSATTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGNGGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRSDIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNAENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSSIFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKVLDSKPSVLALNIQRGDSTIYLLMQ
Purification
Affinity purified using IMAC
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid or Lyophilized powder
Reconstitution
-20°C or -80°C
Molecular Weight
62.8 kDa
Protein Length
Recombinant
NCBI Gene Symbol
DegP
Host or Source
Escherichia coli
Protein Name
Periplasmic serine endoprotease DegP
Gene Name URL
DegP
Nucleotide Accession Number
NC_000913.3

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QP7007-ye-10ugRecombinant E.coli Periplasmic serine endoprotease DegP Protein, His, Yeast-10ug
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QP7007-ye-50ugRecombinant E.coli Periplasmic serine endoprotease DegP Protein, His, Yeast-50ug
QP7007-ye-100ugRecombinant E.coli Periplasmic serine endoprotease DegP Protein, His, Yeast-100ug
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JN944012-01Recombinant Helicobacter pylori HP_1019/Periplasmic serine endoprotease DegP-like Protein, C-His
QP7007-ec-1mgRecombinant E.coli Periplasmic serine endoprotease DegP Protein, His-SUMO, E.coli-1mg

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