NATIVE MUMPS VIRUS Antigen

• Description:

  Mumps virus is a member of the Paramyxoviridae family and is the causative agent of mumps. Mumps is most typically characterised by painful swelling of the salivary glands and fever. In some adult men, the virus might also infect the testicles, rarely causing sterility. The virus is spread by airborne droplets from the nose or throat and can have an incubation period of up to three weeks. Infected individuals are contagious from approximately 6 days before until about 9 days after the onset of symptoms. The disease is self-limiting and treatment involves controlling specific symptoms. An effective vaccine exists and may be given separately or as part of the MMR vaccine

• Reactivity:

  Mumps Virus

• Type:

  Others

• Source:

  Mumps virus, Enders strain, cultured in BSC-1 cells

• Applications:

  Enzyme-linked immunosorbent assay

• Purification:

  Mumps virus, Enders strain, cultured in BSC-1 cells. Optimally infected cells are harvested, disrupted by sonication in culture medium and subjected to low speed centrifugation. The supernatant from the infected culture is concentrated by crossflow ultrafiltration. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Concentration:

  1 mg/ml

• Homology:

  Viral

• Bioactivity:

  Antigenic activity is 51.1% of internal reference batch.

• Format:

  Liquid. Culture medium 1969B

• Reconstitution:

  -80°C

• Notes:

  Formerly GWB-7ED513

• Protein Length:

  Native

• Protein Name:

  Nucleoprotein

• Preparation:

  Optimally infected cells are harvested, disrupted by sonication in culture medium and subjected to low speed centrifugation. The supernatant from the infected culture is concentrated by crossflow ultrafiltration. The antigen preparation is inactivated using gamma radiation, which primarily damages viral genetic material.

• CAS Number:

  9007-83-4