Products for Research Use Only

TMB ONE Accessory Reagent

CAT: 0247-OOSA09597-100MLSize: 100 mLDry Ice: NoHazardous: No
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CAT#:0247-OOSA09597-100MLSize:100 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Target
OOSA09597 is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP) . TMB CORE contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. OOSA09597 produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue) . However, for endpoint assays the reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Type
Others
Applications
Enzyme-linked immunosorbent assay
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Format
Liquid
Reconstitution
2°C to 8°C
Notes
Formerly GWB-Q00244