IL12B ELISA Kit (Rabbit)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Name:

  Interleukin 12B

• Gene Aliases:

  Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL-12 subunit p40; interleukin-12 subunit beta.

• Gene ID:

  100354852

• Accession Number:

  XP_002710393

• Reactivity:

  Oryctolagus cuniculus|Rabbit

• Target:

  Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology IL12b ELISA Kit (Rabbit) (OKWB00410) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL12b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL12b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL12b captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  31.25-2000pg/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 91-97 95 EDTA Plasma (n=5) 88-103 96 Heparin Plasma (n=5) 94-104 99 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 91-97 95 Serum (n=5) 91-97 95 EDTA Plasma (n=5) 88-103 96 EDTA Plasma (n=5) 88-103 96 Heparin Plasma (n=5) 94-104 99 Heparin Plasma (n=5) 94-104 99

• Sensitivity:

  18.75 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount IL12b Microplate 96 Wells (12 x 8 Well strips) IL12b Lyophilized Standard 2 x 2 ng 100X IL12b Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount IL12b Microplate 96 Wells (12 x 8 Well strips) IL12b Microplate 96 Wells (12 x 8 Well strips) IL12b Lyophilized Standard 2 x 2 ng IL12b Lyophilized Standard 2 x 2 ng 100X IL12b Biotinylated Detector Anitbody 1 x 120 uL 100X IL12b Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  IL12B

• Protein Name:

  Interleukin-12 subunit beta

• Gene Name URL:

  IL12B

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  XM_002710347