SLC19A1 ELISA Kit (Rat)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Aliases:

  Reduced folate carrier 1; Solute carrier family 19 member 1.

• Reactivity:

  Rat|Rattus norvegicus

• Target:

  Transporter for the intake of folate, reduced folates and methotrexate.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology Slc19a1 ELISA Kit (Rat) (OKWB00391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc19a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc19a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc19a1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  31.25-2000pg/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 86-99 92 EDTA Plasma (n=5) 89-102 98 Heparin Plasma (n=5) 91-100 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-99 92 Serum (n=5) 86-99 92 EDTA Plasma (n=5) 89-102 98 EDTA Plasma (n=5) 89-102 98 Heparin Plasma (n=5) 91-100 94 Heparin Plasma (n=5) 91-100 94

• Sensitivity:

  18.75 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Lyophilized Standard 2 x 2 ng 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Lyophilized Standard 2 x 2 ng Slc19a1 Lyophilized Standard 2 x 2 ng 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  SLC19A1

• Protein Name:

  Folate transporter 1

• Gene Name URL:

  SLC19A1

• CAS Number:

  7732-18-5