RegisterLogin

SLC19A1 ELISA Kit (Rat)

CAT: 0247-OKWB00391-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKWB00391-96WSize:96 Well
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
Reduced folate carrier 1; Solute carrier family 19 member 1.
Reactivity
Rat|Rattus norvegicus
Target
Transporter for the intake of folate, reduced folates and methotrexate.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Slc19a1 ELISA Kit (Rat) (OKWB00391) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc19a1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc19a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc19a1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
31.25-2000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-99 92 EDTA Plasma (n=5) 89-102 98 Heparin Plasma (n=5) 91-100 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-99 92 Serum (n=5) 86-99 92 EDTA Plasma (n=5) 89-102 98 EDTA Plasma (n=5) 89-102 98 Heparin Plasma (n=5) 91-100 94 Heparin Plasma (n=5) 91-100 94
Sensitivity
18.75 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Lyophilized Standard 2 x 2 ng 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Microplate 96 Wells (12 x 8 Well strips) Slc19a1 Lyophilized Standard 2 x 2 ng Slc19a1 Lyophilized Standard 2 x 2 ng 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Slc19a1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
SLC19A1
Protein Name
Folate transporter 1
Gene Name URL
SLC19A1

Related Products

CatalogName
ARP44167_T100SLC19A1
AAP44167-100UGSLC19A1 Peptide (AAP44167)
OCOA04217-20UGSLC19A1 Protein Lysate
OKEH06197-96WSLC14A1 ELISA Kit (Rat)
OKEH06576-96WSLC12A1 ELISA Kit (Rat)
OKEH01825-96WSLC16A1 ELISA Kit (Rat)
OKEH04285-96WSLC11A2 ELISA Kit (Rat)
OKCD08649-96WSLC11A2 ELISA Kit (Rat)
OABB01987-100UGSLC19A1 Antibody - middle region
OKEH06197SLC14A1 ELISA Kit (Rat) (OKEH06197)

Alternative Products

CatalogName
ELI-15593rRat Slc19a1 ELISA Kit
D4725Folate Transporter 1 (SLC19A1) Rat ELISA Kit
EIA07342rRat Folate transporter 1 (SLC19A1) ELISA Kit
MBS9331917-01Rat Folate transporter 1, SLC19A1 ELISA Kit
MBS7606581-01Rat SLC19A1 (Folate transporter 1) ELISA Kit
KLR4410GENLISA™ Folate transporter 1 (Rat SLC19A1) ELISA
KLR6557GENLISA Rat sLC19A1 (Folate Transporter 1) ELISA
370231-01SLC19A1 (Folate Transporter 1) BioAssayTM ELISA Kit (Rat) discontinued
abx257971-01Rat Solute Carrier Family 19 Member 1 (SLC19A1) ELISA Kit
43919056SLC19A1 Adenovirus (Rat)

Popular Products