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SLC11A2 ELISA Kit (Rat)

CAT: 0247-OKEH04285-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04285-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Solute carrier family 11 member 2
Gene Aliases
Divalent cation transporter 1; divalent metal transporter 1; Dmt1; DMT-1; natural resistance-associated macrophage protein 2; NRAMP 2; Nramp2; solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2; Solute carrier family 11 member 2; Solute carrier family 11 member 2 (natural resistance-associated macrophage protein 2) .
Gene ID
25715
Accession Number
NP_037305.2
Reactivity
Rat, Rattus norvegicus
Target
Natural resistance-associated macrophage protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Slc11a2 ELISA Kit (Rat) (OKEH04285) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Slc11a2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Slc11a2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Slc11a2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
15.6-1000pg/mL
Sensitivity
7.9 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
62 kDa
Specificity
Natural and recombinant Rat Natural resistance-associated macrophage protein 2
Protein Length
568
NCBI Gene Symbol
Slc11a2
Protein Name
O54902
Gene Name URL
Slc11a2
Nucleotide Accession Number
NM_013173.2

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