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ADNP ELISA Kit (Mouse)

CAT: 0247-OKWB00276-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00276-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Activity-dependent neuroprotective protein
Gene Aliases
AA589558; Activity-dependent neuroprotective protein; activity-dependent neuroprotector homeobox protein; mKIAA0784.
Gene ID
11538
Accession Number
NP_001297015
Reactivity
Mouse|Mus musculus
Target
Potential transcription factor. May mediate some of the neuroprotective peptide VIP-associated effects involving normal growth and cancer proliferation.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Adnp ELISA Kit (Mouse) (OKWB00276) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adnp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adnp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adnp captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
31.25-2000pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-104 94 EDTA Plasma (n=5) 85-105 95 Heparin Plasma (n=5) 86-101 93 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-104 94 Serum (n=5) 86-104 94 EDTA Plasma (n=5) 85-105 95 EDTA Plasma (n=5) 85-105 95 Heparin Plasma (n=5) 86-101 93 Heparin Plasma (n=5) 86-101 93
Sensitivity
18.75 pg/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Adnp Microplate 96 Wells (12 x 8 Well strips) Adnp Lyophilized Standard 2 x 2 ng 100X Adnp Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Adnp Microplate 96 Wells (12 x 8 Well strips) Adnp Microplate 96 Wells (12 x 8 Well strips) Adnp Lyophilized Standard 2 x 2 ng Adnp Lyophilized Standard 2 x 2 ng 100X Adnp Biotinylated Detector Anitbody 1 x 120 uL 100X Adnp Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Adnp
Protein Name
Activity-dependent neuroprotector homeobox protein
Gene Name URL
Adnp
Nucleotide Accession Number
NM_001310086

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