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PRL3B1 ELISA Kit (Mouse)

CAT: 0247-OKWB00270-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00270-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Name
Prolactin family 3, subfamily b, member 1
Gene Aliases
Chorionic somatomammotropin hormone 2; Csh; Csh2; Ghd8; growth hormone d8; m; mP; mplII; mPL-II; P; PL; Pl2; Pl-2; placental lactogen 2; placental lactogen II; PL-II; prolactin-3B1.
Gene ID
18776
Accession Number
NP_032891
Reactivity
Mouse|Mus musculus
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric, OD 450 nm
Assay Principle
Aviva Systems Biology Prl3b1 ELISA Kit (Mouse) (OKWB00270) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with Prl3b1. Sample or standards are added to the wells along with a Prl3b1 biotinylated detector antibody and incubated. The Prl3b1 found in the sample or standards inhibits the Prl3b1 detector antibody from binding to the immobilized Prl3b1 on the plate. Excess unbound sample or standard Prl3b1 and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of Prl3b1 biotinylated detector antibody bound in the well and inversely proportional to the amount of Prl3b1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Sample Type Range Mean Serum (n=5) 85-100 91 EDTA Plasma (n=5) 85-102 93 Heparin Plasma (n=5) 90-105 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 85-100 91 Serum (n=5) 85-100 91 EDTA Plasma (n=5) 85-102 93 EDTA Plasma (n=5) 85-102 93 Heparin Plasma (n=5) 90-105 94 Heparin Plasma (n=5) 90-105 94
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Prl3b1 Microplate 96 Wells (12 x 8 Well strips) Prl3b1 Lyophilized Standard 2 x 10 ng 100X Prl3b1 Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Prl3b1 Microplate 96 Wells (12 x 8 Well strips) Prl3b1 Microplate 96 Wells (12 x 8 Well strips) Prl3b1 Lyophilized Standard 2 x 10 ng Prl3b1 Lyophilized Standard 2 x 10 ng 100X Prl3b1 Biotinylated Detector Anitbody 1 x 60 uL 100X Prl3b1 Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
Prl3b1
Protein Name
Prolactin-3B1
Gene Name URL
Prl3b1
Nucleotide Accession Number
NM_008865

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