PROKR1 ELISA Kit (Human)

• Reproducibility:

  Mean Inter-Assay Precision

• Gene Name:

  Prokineticin receptor 1

• Gene Aliases:

  G protein-coupled receptor 73; G protein-coupled receptor ZAQ; GPR73; GPR73a; G-protein coupled receptor 73; G-protein coupled receptor ZAQ; PKR1; PK-R1; prokineticin receptor 1; ZAQ.

• Gene ID:

  10887

• Accession Number:

  NP_620414

• Reactivity:

  Homo sapiens|Human

• Target:

  Receptor for prokineticin 1. Exclusively coupled to the G (q) subclass of heteromeric G proteins. Activation leads to mobilization of calcium, stimulation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. May play a role during early pregnancy.

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Detection Method:

  Colorimetric, OD 450 nm

• Assay Principle:

  Aviva Systems Biology PROKR1 ELISA Kit (Human) (OKWB00181) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PROKR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PROKR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PROKR1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

• Detection Range:

  0.625-40ng/ml

• Recovery:

  Sample Type Range Mean Serum (n=5) 89-101 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 92-102 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 89-101 95 Serum (n=5) 89-101 95 EDTA Plasma (n=5) 89-102 95 EDTA Plasma (n=5) 89-102 95 Heparin Plasma (n=5) 92-102 96 Heparin Plasma (n=5) 92-102 96

• Sensitivity:

  0.375 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Components:

  Component Amount PROKR1 Microplate 96 Wells (12 x 8 Well strips) PROKR1 Lyophilized Standard 2 x 40 ng 100X PROKR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount PROKR1 Microplate 96 Wells (12 x 8 Well strips) PROKR1 Microplate 96 Wells (12 x 8 Well strips) PROKR1 Lyophilized Standard 2 x 40 ng PROKR1 Lyophilized Standard 2 x 40 ng 100X PROKR1 Biotinylated Detector Anitbody 1 x 120 uL 100X PROKR1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

• NCBI Gene Symbol:

  PROKR1

• Protein Name:

  Prokineticin receptor 1

• Gene Name URL:

  PROKR1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_138964