NEB ELISA Kit (Human)

Mean Inter-Assay Precision

7732-18-5

NEM2, NEB177D

4703

NP_004534

Human

This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.

ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Colorimetric, OD 450 nm

Aviva Systems Biology NEB ELISA Kit (Human) (OKWB00178) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with NEB. Sample or standards are added to the wells along with a NEB biotinylated detector antibody and incubated. The NEB found in the sample or standards inhibits the NEB detector antibody from binding to the immobilized NEB on the plate. Excess unbound sample or standard NEB and detector antibody is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of NEB biotinylated detector antibody bound in the well and inversely proportional to the amount of NEB which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

6.25-400ng/ml

Sample Type Range Mean Serum (n=5) 86-98 92 EDTA Plasma (n=5) 85-104 94 Heparin Plasma (n=5) 88-105 98 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-98 92 Serum (n=5) 86-98 92 EDTA Plasma (n=5) 85-104 94 EDTA Plasma (n=5) 85-104 94 Heparin Plasma (n=5) 88-105 98 Heparin Plasma (n=5) 88-105 98

3.75 ng/mL

Store as indicated in product manual.

Component Amount NEB Microplate 96 Wells (12 x 8 Well strips) NEB Lyophilized Standard 2 x 400 ng 100X NEB Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount NEB Microplate 96 Wells (12 x 8 Well strips) NEB Microplate 96 Wells (12 x 8 Well strips) NEB Lyophilized Standard 2 x 400 ng NEB Lyophilized Standard 2 x 400 ng 100X NEB Biotinylated Detector Anitbody 1 x 60 uL 100X NEB Biotinylated Detector Anitbody 1 x 60 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

NEB

Nebulin

NEB