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DHPS ELISA Kit (Human)

CAT: 0247-OKWB00169-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00169-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
DS, DHS, MIG13, NEDSSWI
Gene ID
1725
Accession Number
NP_001193903
Reactivity
Human
Target
Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology DHPS ELISA Kit (Human) (OKWB00169) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DHPS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DHPS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DHPS captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
0.313 - 20 ng/mL
Recovery
Sample Type Range Mean Serum (n=5) 87-99 92 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 95 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 87-99 92 Serum (n=5) 87-99 92 EDTA Plasma (n=5) 86-105 96 EDTA Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 85-104 95 Heparin Plasma (n=5) 85-104 95
Sensitivity
0.188 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
DHPS
Protein Name
Deoxyhypusine synthase
Gene Name URL
DHPS
Nucleotide Accession Number
NM_001206974

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