ZC3H12A ELISA Kit (Human)

Mean Inter-Assay Precision

7732-18-5

Zinc finger CCCH-type containing 12A

Bifunctional endoribonuclease and deubiquitinase ZC3H12A; dJ423B22.1; endoribonuclease ZC3H12A; MCP-1 treatment-induced protein; MCP-induced protein 1; MCPIP; MCPIP1; MCPIP-1; monocyte chemotactic protein-induced protein 1; Reg1; regnase-1; ribonuclease ZC3H12A; zinc finger CCCH domain-containing protein 12A.

80149

NP_001310479

Homo sapiens|Human

Endoribonuclease involved in various biological functions such as cellular inflammatory response and immune homeostasis, glial differentiation of neuroprogenitor cells, cell death of cardiomyocytes, adipogenesis and angiogenesis. Functions as an endoribonuclease involved in mRNA decay (PubMed:19909337) . Modulates the inflammatory response by promoting the degradation of a set of translationally active cytokine-induced inflammation-related mRNAs, such as IL6 and IL12B, during the early phase of inflammation (PubMed:26320658) . Prevents aberrant T-cell-mediated immune reaction by degradation of multiple mRNAs controlling T-cell activation, such as those encoding cytokines (IL6 and IL2), cell surface receptors (ICOS, TNFRSF4 and TNFR2) and transcription factor (REL) (By similarity) . Inhibits cooperatively with ZC3H12A the differentiation of helper T cells Th17 in lungs. They repress target mRNA encoding the Th17 cell-promoting factors IL6, ICOS, REL, IRF4, NFKBID and NFKBIZ. The cooperation requires RNA-binding by RC3H1 and the nuclease activity of ZC3H12A (By similarity) . Self regulates by destabilizing its own mRNA (By similarity) . Cleaves mRNA harboring a stem-loop (SL), often located in their 3'-UTRs, during the early phase of inflammation in a helicase UPF1-dependent manner (PubMed:19909337, PubMed:26320658, PubMed:26134560, PubMed:22561375) . Plays a role in the inhibition of microRNAs (miRNAs) biogenesis (PubMed:22055188) . Cleaves the terminal loop of a set of precursor miRNAs (pre-miRNAs) important for the regulation of the inflammatory response leading to their degradation, and thus preventing the biosynthesis of mature miRNAs (PubMed:22055188) . Plays also a role in promoting angiogenesis in response to inflammatory cytokines by inhibiting the production of antiangiogenic microRNAs via its anti-dicer RNase activity (PubMed:24048733) . Affects the overall ubiquitination of cellular proteins (By similarity) . Positively regulates deubiquitinase activity promoting the cleavage at 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains on TNF receptor-associated factors (TRAFs), preventing JNK and NF-kappa-B signaling pathway activation, and hence negatively regulating macrophage-mediated inflammatory response and immune homeostasis (By similarity) . Induces also deubiquitination of the transcription factor HIF1A, probably leading to its stabilization and nuclear import, thereby positively regulating the expression of proangiogenic HIF1A-targeted genes (PubMed:24048733) . Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage (PubMed:25861989) . Prevents stress granule (SGs) formation and promotes macrophage apoptosis under stress conditions, including arsenite-induced oxidative stress, heat shock and energy deprivation (By similarity) . Plays a role in the regulation of macrophage polarization; promotes IL4-induced polarization of macrophages M1 into anti-inflammatory M2 state (By similarity) . May also act as a transcription factor that regulates the expression of multiple genes involved in inflammatory response, angiogenesis, adipogenesis and apoptosis (PubMed:16574901, PubMed:18364357) . Functions as a positive regulator of glial differentiation of neuroprogenitor cells through an amyloid precursor protein (APP) -dependent signaling pathway (PubMed:19185603) . Attenuates septic myocardial contractile dysfunction in response to lipopolysaccharide (LPS) by reducing I-kappa-B-kinase (IKK) -mediated NF-kappa-B activation, and hence myocardial proinflammatory cytokine production (By similarity) .

ELISA Kit

Enzyme-linked Immunosorbent assay-Sandwich

Colorimetric, OD 450 nm

Aviva Systems Biology ZC3H12A ELISA Kit (Human) (OKWB00161) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZC3H12A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZC3H12A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZC3H12A captured in the well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

3.906-250pg/ml

Sample Type Range Mean Serum (n=5) 89-102 95 EDTA Plasma (n=5) 94-105 99 Heparin Plasma (n=5) 86-103 94 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 89-102 95 Serum (n=5) 89-102 95 EDTA Plasma (n=5) 94-105 99 EDTA Plasma (n=5) 94-105 99 Heparin Plasma (n=5) 86-103 94 Heparin Plasma (n=5) 86-103 94

2.34 pg/mL

Store as indicated in product manual.

Component Amount ZC3H12A Microplate 96 Wells (12 x 8 Well strips) ZC3H12A Lyophilized Standard 2 x 250 pg 100X ZC3H12A Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount ZC3H12A Microplate 96 Wells (12 x 8 Well strips) ZC3H12A Microplate 96 Wells (12 x 8 Well strips) ZC3H12A Lyophilized Standard 2 x 250 pg ZC3H12A Lyophilized Standard 2 x 250 pg 100X ZC3H12A Biotinylated Detector Anitbody 1 x 120 uL 100X ZC3H12A Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL

ZC3H12A

Endoribonuclease ZC3H12A

ZC3H12A