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MT ELISA Kit (Chicken)

CAT: 0247-OKWB00126-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKWB00126-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision
CAS Number
7732-18-5
Gene Aliases
MT2A, MT4L
Gene ID
396212
Accession Number
NP_990606
Reactivity
Chicken
Target
Metallothioneins have a high content of cysteine residues that bind various heavy metals.
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Detection Method
Colorimetric, OD 450 nm
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Detection Range
7.813-500pg/ml
Recovery
Sample Type Range Mean Serum (n=5) 86-105 95 EDTA Plasma (n=5) 90-105 98 Heparin Plasma (n=5) 86-105 96 Sample Type Range Mean Sample Type Range Mean Serum (n=5) 86-105 95 Serum (n=5) 86-105 95 EDTA Plasma (n=5) 90-105 98 EDTA Plasma (n=5) 90-105 98 Heparin Plasma (n=5) 86-105 96 Heparin Plasma (n=5) 86-105 96
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
Components
Component Amount Mt Microplate 96 Wells (12 x 8 Well strips) Mt Lyophilized Standard 2 x 10 ng 100X Mt Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Component Amount Component Amount Mt Microplate 96 Wells (12 x 8 Well strips) Mt Microplate 96 Wells (12 x 8 Well strips) Mt Lyophilized Standard 2 x 10 ng Mt Lyophilized Standard 2 x 10 ng 100X Mt Biotinylated Detector Anitbody 1 x 120 uL 100X Mt Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 25X Wash Buffer 1 x 20 mL 25X Wash Buffer 1 x 20 mL TMB Substrate 1 x 10 mL TMB Substrate 1 x 10 mL Stop Solution 1 x 10 mL Stop Solution 1 x 10 mL
NCBI Gene Symbol
MT
Protein Name
Metallothionein
Gene Name URL
MT
Nucleotide Accession Number
NM_205275

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