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MT-ND5 ELISA Kit (Human)

CAT: 0247-OKEH04747-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04747-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
NADH dehydrogenase subunit 5
Gene Aliases
MTND5; NADH dehydrogenase subunit 5.
Gene ID
4540
Accession Number
YP_003024036.1
Reactivity
Homo sapiens, Human
Target
NADH-ubiquinone oxidoreductase chain 5
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MT-ND5 ELISA Kit (Human) (OKEH04747) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MT-ND5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MT-ND5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MT-ND5 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.165 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
67 kDa
Specificity
Natural and recombinant Human NADH-ubiquinone oxidoreductase chain 5
Protein Length
603
NCBI Gene Symbol
ND5
Protein Name
P03915
Gene Name URL
ND5
Nucleotide Accession Number
NC_012920.1

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