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GRK2 ELISA Kit (Rat)

CAT: 0247-OKEI00913-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEI00913-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
BARK1, GRK-2, Adrbk1
Gene ID
25238
Accession Number
NP_036908
Reactivity
Rat
Target
Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Grk2 ELISA Kit (Rat) (OKEI00913) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grk2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Grk2
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.156 - 10 ng/mL
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
GRK2
Protein Name
Beta-adrenergic receptor kinase 1
Gene Name URL
GRK2
Nucleotide Accession Number
NM_012776

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