CENPH ELISA Kit (Mouse)

CAT: 0247-OKEI00398-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEI00398-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Aliases
E, ENP, CENP, CENP-H, AU044255, 1700021I11Rik, 2410018A12Rik, 2610042E16Rik, 2810046K12Rik
Gene ID
26886
Accession Number
NP_068686
Reactivity
Mouse
Target
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Cenph ELISA Kit (Mouse) (OKEI00398) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cenph has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cenph is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Cenph
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Biological Fluids
Detection Range
0.078 - 5 ng/mL
Sensitivity
46.9 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CENPH
Protein Name
Centromere protein H
Gene Name URL
CENPH
Nucleotide Accession Number
NM_021886

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