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VPS29 ELISA Kit (Human)

CAT: 0247-OKEH08609-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08609-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
VPS29 retromer complex component
Gene Aliases
DC15; DC7; epididymis secretory sperm binding protein; hVPS29; PEP11; PEP11 homolog; retromer protein; vacuolar protein sorting 29 homolog; vacuolar protein sorting-associated protein 29; vacuolar sorting protein VPS29/PEP11; vesicle protein sorting 29; x 007 protein.
Gene ID
51699
Accession Number
NP_057310.1
Reactivity
Homo sapiens, Human
Target
Vacuolar protein sorting-associated protein 29
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology VPS29 ELISA Kit (Human) (OKEH08609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VPS29 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for VPS29 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VPS29 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87% (n = 20)
Sensitivity
23pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Vacuolar protein sorting-associated protein 29
NCBI Gene Symbol
VPS29
Protein Name
Q9UBQ0
Gene Name URL
VPS29
Nucleotide Accession Number
NM_016226.4

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