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TRIM63 ELISA Kit (Rat)

CAT: 0247-OKEH08601-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#: OKEH08601-96WSize: 96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Tripartite motif containing 63
Gene Aliases
E3 ubiquitin-protein ligase TRIM63; Murf; Murf1; MURF-1; muscle ring finger protein 1; muscle-specific RING finger protein 1; ring finger protein 28; RING-type E3 ubiquitin transferase TRIM63; Rnf28; tripartite motif containing 63, E3 ubiquitin protein ligase; tripartite motif protein 63; tripartite motif-containing protein 63.
Gene ID
140939
Accession Number
NP_543179.1
Reactivity
Rat, Rattus norvegicus
Target
E3 ubiquitin-protein ligase TRIM63
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TRIM63 ELISA Kit (Rat) (OKEH08601) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRIM63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM63 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
Sensitivity
0.157ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat E3 ubiquitin-protein ligase TRIM63
NCBI Gene Symbol
Trim63
Protein Name
Q91Z63
Gene Name URL
Trim63
Nucleotide Accession Number
NM_080903.1

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