TRIM63 ELISA Kit (Rat)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Tripartite motif containing 63

• Gene Aliases:

  E3 ubiquitin-protein ligase TRIM63; Murf; Murf1; MURF-1; muscle ring finger protein 1; muscle-specific RING finger protein 1; ring finger protein 28; RING-type E3 ubiquitin transferase TRIM63; Rnf28; tripartite motif containing 63, E3 ubiquitin protein ligase; tripartite motif protein 63; tripartite motif-containing protein 63.

• Gene ID:

  140939

• Accession Number:

  NP_543179.1

• Reactivity:

  Rat, Rattus norvegicus

• Target:

  E3 ubiquitin-protein ligase TRIM63

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology TRIM63 ELISA Kit (Rat) (OKEH08601) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TRIM63 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TRIM63 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TRIM63 captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)

• Sensitivity:

  0.157ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Rat E3 ubiquitin-protein ligase TRIM63

• NCBI Gene Symbol:

  Trim63

• Protein Name:

  Q91Z63

• Gene Name URL:

  Trim63

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_080903.1