MSMP ELISA Kit (Human)

• Reproducibility:

  Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //

• Gene Name:

  Microseminoprotein, prostate associated

• Gene Aliases:

  PC3 prostate cancer cell-secreted microprotein; PC3-secreted microprotein; prostate-associated microseminoprotein; PSMP.

• Gene ID:

  692094

• Accession Number:

  NP_001037729.1

• Reactivity:

  Homo sapiens, Human

• Target:

  Prostate-associated microseminoprotein

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology MSMP ELISA Kit (Human) (OKEH08352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MSMP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MSMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MSMP captured in well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)

• Sensitivity:

  0.085ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Human Prostate-associated microseminoprotein

• NCBI Gene Symbol:

  MSMP

• Protein Name:

  Q1L6U9

• Gene Name URL:

  MSMP

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001044264.2