MSMB ELISA Kit (Rat)
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MSMB ELISA Kit (Rat)
Reproducibility :
Mean Inter-Assay Precision -Gene Name :
Microseminoprotein, betaGene Aliases :
Beta-microseminoprotein; prostate secreted seminal plasma protein; prostate secretory protein of 94 amino acids; prostate secretory protein PSP94; PSP94; PSP-94.Gene ID :
29311Accession Number :
NP_062061.1Reactivity :
Rat, Rattus norvegicusTarget :
Beta-microseminoproteinType :
ELISA KitApplications :
Enzyme-linked Immunosorbent assay-SandwichAssay Principle :
Aviva Systems Biology Msmb ELISA Kit (Rat) (OKEH05993) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Msmb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Msmb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Msmb captured in well.Assay Protocol :
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type :
Serum, plasma and other biological fluidsDetection Range :
0.312-20ng/mLRecovery :
Mean recovery when spiking into Serum and Plasma = 97%Sensitivity :
0.161 ng/mLReconstitution :
Store as indicated in product manual.Specificity :
Natural and recombinant Rat Beta-microseminoproteinProtein Length :
113NCBI Gene Symbol :
MsmbProtein Name :
P97580Gene Name URL :
MsmbCAS Number :
7732-18-5Nucleotide Accession Number :
NM_019188.1

