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B9D1 ELISA Kit (Human)

CAT: 0247-OKEH08336-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08336-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
B9 domain containing 1
Gene Aliases
B9; B9 domain-containing protein 1; B9 protein domain 1; endothelial precursor protein B9; EPPB9; JBTS27; MKS1-related protein 1; MKS9; MKSR1; MKSR-1.
Gene ID
27077
Accession Number
NP_001308145.1
Reactivity
Homo sapiens, Human
Target
B9 domain-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology B9D1 ELISA Kit (Human) (OKEH08336) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B9D1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B9D1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B9D1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
Sensitivity
0.085ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human B9 domain-containing protein 1
NCBI Gene Symbol
B9D1
Protein Name
Q9UPM9
Gene Name URL
B9D1
Nucleotide Accession Number
NM_001321216.1

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