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SNCAIP ELISA Kit (Mouse)

CAT: 0247-OKEH08286-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08286-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Synuclein, alpha interacting protein (synphilin)
Gene Aliases
2810407O15Rik;4933427B05Rik; alpha-synuclein-interacting protein; BB104594; synp; synphilin-1; SYP; SYPH1.
Gene ID
67847
Accession Number
NP_001186080.1
Reactivity
Mouse, Mus musculus
Target
Synphilin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SNCAIP ELISA Kit (Mouse) (OKEH08286) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNCAIP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNCAIP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNCAIP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20)
Sensitivity
34pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Synphilin-1
NCBI Gene Symbol
Sncaip
Protein Name
Q99ME3
Gene Name URL
Sncaip
Nucleotide Accession Number
NM_001199151.1

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