CPS1 ELISA Kit (Mouse)

CAT: 0247-OKEH08131-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH08131-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Carbamoyl-phosphate synthetase 1
Gene Aliases
4732433M03Rik; C; carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoylphosphate synthetase I; carbamoyl-phosphate synthetase I; CPS; CPSase I; D1Ucl; D1Ucla3.
Gene ID
227231
Accession Number
NP_001074278.1
Reactivity
Mouse, Mus musculus
Target
Carbamoyl-phosphate synthase [ammonia], mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CPS1 ELISA Kit (Mouse) (OKEH08131) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPS1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
31.2-2000 pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
Sensitivity
7.9pg/ml
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Carbamoyl-phosphate synthase [ammonia], mitochondrial
NCBI Gene Symbol
Cps1
Protein Name
Q8C196
Gene Name URL
Cps1
Nucleotide Accession Number
NM_001080809.2

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