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SNTN ELISA Kit (Mouse)

CAT: 0247-OKEH08048-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH08048-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Sentan, cilia apical structure protein
Gene Aliases
A430083B19Rik; Protein S100-A1-like; S100 calcium-binding protein A1-like; sentan.
Gene ID
218739
Accession Number
NP_808292.1
Reactivity
Mouse, Mus musculus
Target
Sentan
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SNTN ELISA Kit (Mouse) (OKEH08048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SNTN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SNTN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SNTN captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.156-10 ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 118% (n = 20)
Sensitivity
0.067 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Sentan
NCBI Gene Symbol
Sntn
Protein Name
Q8C9X1
Gene Name URL
Sntn
Nucleotide Accession Number
NM_177624.3

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