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WT1 ELISA Kit (Pig)

CAT: 0247-OKEH07744-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07744-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
WT1 transcription factor
Gene Aliases
Wilms tumor 1; Wilm's tumor protein 1; Wilms tumor protein homolog; WT-1.
Gene ID
397338
Accession Number
NP_001001264.1
Reactivity
Porcine, Sus scrofa
Target
Wilms tumor protein homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WT1 ELISA Kit (Pig) (OKEH07744) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WT1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WT1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WT1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85% (n = 20)
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Pig Wilms tumor protein homolog
NCBI Gene Symbol
WT1
Protein Name
O62651
Gene Name URL
WT1
Nucleotide Accession Number
NM_001001264.1

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