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WT1 ELISA Kit (Mouse)

CAT: 0247-OKEH03610-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH03610-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Wilms tumor 1 homolog
Gene Aliases
Wt-1, D630046I19Rik
Gene ID
22431
Reactivity
Mouse, Mus musculus
Target
Wilms tumor protein homolog
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WT1 ELISA Kit (Mouse) (OKEH03610) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Wt1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Wt1 and incubated. The Wt1 found in the sample or standards competes with the biotinylated Wt1 for limited binding sites on the immobilized anti-human Wt1 antibody. Excess unbound biotinylated Wt1 and sample or standard Wt1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Wt1 captured in the well and inversely proportional to the amount of Wt1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
39.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 kDa
Specificity
Natural and recombinant Mouse Wilms tumor protein homolog
Protein Length
305
NCBI Gene Symbol
WT1
Protein Name
P22561
Gene Name URL
WT1

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