MAP4K1 ELISA Kit (Human)

CAT: 0247-OKEH07589-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH07589-96WSize:96 Tests
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase kinase kinase kinase 1
Gene Aliases
Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; mitogen-activated protein kinase kinase kinase kinase 1.
Gene ID
11184
Accession Number
NP_001036065.1
Reactivity
Homo sapiens, Human
Target
Mitogen-activated protein kinase kinase kinase kinase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAP4K1 ELISA Kit (Human) (OKEH07589) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAP4K1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAP4K1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAP4K1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
Sensitivity
38pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Mitogen-activated protein kinase kinase kinase kinase 1
NCBI Gene Symbol
MAP4K1
Protein Name
Q92918
Gene Name URL
MAP4K1
Nucleotide Accession Number
NM_001042600.2

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