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HDLC ELISA Kit (General)

CAT: 0247-OKEH07537-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07537-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Reactivity
All Species
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology HDLC ELISA Kit (OKEH07537) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-HDLC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HDLC and incubated. The HDLC found in the sample or standards competes with the biotinylated HDLC for limited binding sites on the immobilized anti-HDLC antibody. Excess unbound biotinylated HDLC and sample or standard HDLC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HDLC captured in the well and inversely proportional to the amount of HDLC which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
12.5-800ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84% (n = 20)
Sensitivity
8.5ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant General HDL-cholesterol
NCBI Gene Symbol
HDLC
Protein Name
HDL-cholesterol
Gene Name URL
HDLC

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