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5METHF ELISA Kit (General)

CAT: 0247-OKEH04793-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04793-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Aliases
5-Methyltetrahydrofolate
Reactivity
All Species
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology 5METHF ELISA Kit (General) (OKEH04793) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-general 5METHF antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 5METHF and incubated. The 5METHF found in the sample or standards competes with the biotinylated 5METHF for limited binding sites on the immobilized anti-general 5METHF antibody. Excess unbound biotinylated 5METHF and sample or standard 5METHF is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 5METHF captured in the well and inversely proportional to the amount of 5METHF which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
KDa
Specificity
Natural and recombinant General Levomefolic acid
NCBI Gene Symbol
5METHF
Gene Name URL
5METHF

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