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NPPC ELISA Kit (Chicken)

CAT: 0247-OKEH07460-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07460-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Aliases
CNP
Reactivity
Chicken, Gallus gallus
Target
C-type natriuretic peptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology NPPC ELISA Kit (OKEH07460) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-NPPC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NPPC and incubated. The NPPC found in the sample or standards competes with the biotinylated NPPC for limited binding sites on the immobilized anti-NPPC antibody. Excess unbound biotinylated NPPC and sample or standard NPPC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NPPC captured in the well and inversely proportional to the amount of NPPC which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81% (n = 20)
Sensitivity
15.66pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken C-type natriuretic peptide
NCBI Gene Symbol
NPPC
Protein Name
P21805
Gene Name URL
NPPC

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