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NPPC ELISA Kit (Rat)

CAT: 0247-OKEH04369-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04369-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Natriuretic peptide C
Gene Aliases
C-type natriuretic peptide; natriuretic peptide precursor C; natriuretic peptide precursor type C.
Gene ID
114593
Accession Number
NP_446202.1
Reactivity
Rat, Rattus norvegicus
Target
C-type natriuretic peptide
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology Nppc ELISA Kit (Rat) (OKEH04369) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nppc has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Nppc is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nppc captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
7.8-500pg/mL
Sensitivity
4.23 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 KDa
Specificity
Natural and recombinant Rat C-type natriuretic peptide
Protein Length
126
NCBI Gene Symbol
Nppc
Protein Name
P55207
Gene Name URL
Nppc
Nucleotide Accession Number
NM_053750.1

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