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MBP ELISA Kit (Chicken)

CAT: 0247-OKEH07420-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07420-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Intra-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //Mean Inter-assay CV%: //
CAS Number
7732-18-5
Gene Name
Myelin basic protein
Gene Aliases
Myelin basic protein.
Gene ID
396217
Accession Number
NP_990611.1
Reactivity
Chicken, Gallus gallus
Target
Myelin basic protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology MBP ELISA Kit (OKEH07420) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-MBP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MBP and incubated. The MBP found in the sample or standards competes with the biotinylated MBP for limited binding sites on the immobilized anti-MBP antibody. Excess unbound biotinylated MBP and sample or standard MBP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MBP captured in the well and inversely proportional to the amount of MBP which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
Sensitivity
0.2ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Chicken Myelin basic protein
NCBI Gene Symbol
MBP
Protein Name
P15720
Gene Name URL
MBP
Nucleotide Accession Number
NM_205280.1

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