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MBP ELISA Kit (Rat)

CAT: 0247-OKCA02242-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCA02242-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Reactivity
Rat
Target
Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology MBP ELISA Kit (Rat) (OKCA02242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MBP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MBP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 90 85-96 EDTA plasma (n=4) 95 92-97 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 90 85-96 Serum (n=5) 90 85-96 EDTA plasma (n=4) 95 92-97 EDTA plasma (n=4) 95 92-97
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
MBP
Protein Name
Myelin basic protein
Gene Name URL
MBP

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