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TGFBR2 ELISA Kit (Rat)

CAT: 0247-OKEH07183-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH07183-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Transforming growth factor, beta receptor 2
Gene Aliases
TbetaR-II; TGF-beta 2; TGF-beta receptor type II; TGF-beta receptor type-2; TGF-beta type II receptor; Tgfbr2T; TGFR-2; transforming growth factor beta receptor type II; transforming growth factor, beta receptor II; transforming growth factor, beta receptor IIT; transforming growth factor-b type II receptor; Transforming growth factor-beta receptor type II; transforming growth factor-beta type II receptor.
Gene ID
81810
Accession Number
NP_112394
Reactivity
Rat, Rattus norvegicus
Target
TGF-beta receptor type-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tgfbr2 ELISA Kit (Rat) (OKEH07183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tgfbr2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tgfbr2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tgfbr2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 90%
Sensitivity
0.172 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat TGF-beta receptor type-2
NCBI Gene Symbol
Tgfbr2
Protein Name
P38438
Gene Name URL
Tgfbr2
Nucleotide Accession Number
NM_031132

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