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PRDX6 ELISA Kit (Mouse)

CAT: 0247-OKEH07122-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07122-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Peroxiredoxin 6
Gene Aliases
1-cys;1-Cys peroxiredoxin;1-Cys Prx;1-cysPrx;9430088D19Rik; a; AA690119; acidic calcium-independent phospholipase A2; aiPLA2; antioxidant protein 2; anti-oxidant protein 2, related sequence 3; Aop2; Brp-; Brp-12; CP-; CP-3; glutathione-dependent peroxiredoxin; GP; GPx; LPC acyltransferase 5; LPCAT-5; Ltw; Ltw-; Ltw4; Ltw-4; Lvtw; Lvtw-4; lyso-PC acyltransferase 5; lysophosphatidylcholine acyltransferase 5; N; non-selenium glutathione peroxidase; NSGPx; ORF06; peroxiredoxin 5, related sequence 3; peroxiredoxin 6, related sequence 3; peroxiredoxin-6; Prdx5.
Gene ID
11758
Accession Number
NP_031479
Reactivity
Mouse, Mus musculus
Target
Peroxiredoxin-6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Prdx6 ELISA Kit (Mouse) (OKEH07122) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prdx6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prdx6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prdx6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into matrix = 86.3%
Sensitivity
31.25 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Peroxiredoxin-6
NCBI Gene Symbol
Prdx6
Protein Name
O08709
Gene Name URL
Prdx6
Nucleotide Accession Number
NM_007453