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PDGFA ELISA Kit (Rat)

CAT: 0247-OKEH07102-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH07102-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Platelet derived growth factor subunit A
Gene Aliases
PDGF subunit A; PDGF-1; PDGFACP; platelet derived growth factor, alpha; Platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide; platelet-derived growth factor subunit A.
Gene ID
25266
Accession Number
NP_036933
Reactivity
Rat, Rattus norvegicus
Target
Platelet-derived growth factor subunit A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pdgfa ELISA Kit (Rat) (OKEH07102) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdgfa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pdgfa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdgfa captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 92%
Sensitivity
0.172 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Rat Platelet-derived growth factor subunit A
NCBI Gene Symbol
Pdgfa
Protein Name
P28576
Gene Name URL
Pdgfa
Nucleotide Accession Number
NM_012801

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