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PDGFA ELISA Kit (Rabbit)

CAT: 0247-OKCD01516-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKCD01516-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit A (PDGFA) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Aliases
PDGF-1; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide.
Gene ID
100352388
Accession Number
XP_008251887.1
Reactivity
Oryctolagus cuniculus|Rabbit
Target
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity) .
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Detection Method
Colorimetric
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor Subunit A (PDGFA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor Subunit A (PDGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Platelet Derived Growth Factor Subunit A (PDGFA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2,000pg/mL
Recovery
Matrix Recovery Range (%) Average (%) Serum (n=5) 97-105 101 EDTA Plasma (n=5) 84-102 98 Heparin Plasma (n=5) 86-104 101 Matrix Recovery Range (%) Average (%) Matrix Recovery Range (%) Average (%) Serum (n=5) 97-105 101 Serum (n=5) 97-105 101 EDTA Plasma (n=5) 84-102 98 EDTA Plasma (n=5) 84-102 98 Heparin Plasma (n=5) 86-104 101 Heparin Plasma (n=5) 86-104 101
Sensitivity
< 10.8 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Anti-PDGFA Microplate 96 Wells (12 x 8 Well Strips) PDGFA Lyophilized Standard 2 x 2 ng 100X Biotinylated PDGFA Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount Anti-PDGFA Microplate 96 Wells (12 x 8 Well Strips) Anti-PDGFA Microplate 96 Wells (12 x 8 Well Strips) PDGFA Lyophilized Standard 2 x 2 ng PDGFA Lyophilized Standard 2 x 2 ng 100X Biotinylated PDGFA Detector Antibody 120 uL 100X Biotinylated PDGFA Detector Antibody 120 uL Avidin/HRP Conjugate 120 uL Avidin/HRP Conjugate 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL
Notes
Formerly GWB-KBBWM3
Specificity
This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit A (PDGFA) . No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit A (PDGFA) and analogues was observed.
Protein Length
213
NCBI Gene Symbol
PDGFA
Protein Name
Platelet-derived growth factor subunit A
Gene Name URL
PDGFA
Nucleotide Accession Number
XM_008253665.1

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