Products for Research Use Only

HSPA8 ELISA Kit (Mouse)

CAT: 0247-OKEH07001-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH07001-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Inter-Assay CV
CAS Number
7732-18-5
Gene Name
Heat shock protein 8
Gene Aliases
2410008N15Rik; heat shock 70 kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock protein cognate 70; Hsc7; Hsc70; Hsc71; Hsc73; Hsp; Hsp73; Hspa10.
Gene ID
15481
Accession Number
NP_112442
Reactivity
Mouse, Mus musculus
Target
Heat shock cognate 71 kDa protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hspa8 ELISA Kit (Mouse) (OKEH07001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hspa8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hspa8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hspa8 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into matrix = 102%
Sensitivity
0.167 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Heat shock cognate 71 kDa protein
NCBI Gene Symbol
Hspa8
Protein Name
P63017
Gene Name URL
Hspa8
Nucleotide Accession Number
NM_031165