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HSPA8 ELISA Kit (Human)

CAT: 0247-OKEH04446-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04446-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Heat shock protein family A (Hsp70) member 8
Gene Aliases
Constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 8; heat shock 70kd protein 10; heat shock 70kDa protein 8; heat shock cognate 71 kDa protein; heat shock cognate protein 54; HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP1; LAP-1; lipopolysaccharide-associated protein 1; LPS-associated protein 1; NIP71; N-myristoyltransferase inhibitor protein 71.
Gene ID
3312
Accession Number
NP_006588.1
Reactivity
Homo sapiens, Human
Target
Heat shock cognate 71 kDa protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HSPA8 ELISA Kit (Human) (OKEH04446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for HSPA8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA8 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
71 KDa
Specificity
Natural and recombinant Human Heat shock cognate 71 kDa protein
Protein Length
646
NCBI Gene Symbol
HSPA8
Protein Name
P11142
Gene Name URL
HSPA8
Nucleotide Accession Number
NM_006597.5

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